rabbit polyclonal antibody against col4a1  (Boster Bio)

Journal: Brain Communications

Article Title: ‘Raisin bread sign’ feature of pontine autosomal dominant microangiopathy and leukoencephalopathy

doi: 10.1093/braincomms/fcad281

Figure Lengend Snippet: The pedigree charts of three PADMAL families (Family 1–3) and the identified variant in COL4A1 3′-untranslated region (UTR). ( A – C ) Square symbols indicate men and round symbols indicate women. Closed symbols indicate affected individuals proven by clinical histories or MRI. Diagonal lines indicate deceased individuals. Open symbols indicate individuals without PADMAL proven clinically or radiologically. Arrows indicate probands. The pedigree charts of F1 ( A ) and F2 ( B ) show autosomal dominant inheritance pattern while F3 ( C ) does not. ( D ) Sanger sequencing was performed with the proband (F1-IV-2) in F1. The heterozygous variant of COL4A1 located in c.*33T > A was identified, which is indicated by an arrowhead. ( E ) The healthy control in F1 (F1-IV-1) showed no variant in COL4A1 3′UTR in Sanger sequencing.

Article Snippet: Immunohistochemistry was performed with rabbit polyclonal antibody against COL4A1 (1:100, SAB4300825, Sigma-Aldrich, St Louis, MO) and α-smooth muscle actin (1:250, ab7817, Abcam, Cambridge, MA) through the use of a Ventana BenchMark GX automated slide staining system (Ventana Medical Systems, Tucson, AZ) in accordance with the instructions of the manufacturer.

Techniques: Variant Assay, Sequencing

Journal: Brain Communications

Article Title: ‘Raisin bread sign’ feature of pontine autosomal dominant microangiopathy and leukoencephalopathy

doi: 10.1093/braincomms/fcad281

Figure Lengend Snippet: Histopathology in F1-IV-3. ( A , B ) Gross finding of the brain of F1-IV-2. Atrophies of frontotemporal lobes and brainstem, and the atherosclerosis of the basilar artery were observed. ( C ) The focused image of the pons on fluid attenuated inversion recovery. ( D, E ) Haematoxylin and eosin staining ( D ) and Klüver–Barrera staining ( E ) sections of the pons showed multiple infarctions and existing lesions revealed by MRI. ( F ) Hyalinized vessels in the non-ischaemic lesions had thickened media tunica. ( G ) The pontine ischaemic lesions did not include vessels with thick media tunica. ( H–J ) Vessels in ischaemic lesions ( H ) showed immunopositivity of COL4A1 ( I ) and α-smooth muscle actin ( J ) around the vessels. ( K–M ) Vessels in the non-ischaemic areas ( K ) was hyalinized with thick media tunica, which were immunopositive for COL4A1 ( L ) and α-smooth muscle actin ( M ). Scale bars: ( D, E ) 1 mm, ( F, G ) 200 μm, ( H–J, L, M ) 20 μm, ( K ) 100 μm.

Article Snippet: Immunohistochemistry was performed with rabbit polyclonal antibody against COL4A1 (1:100, SAB4300825, Sigma-Aldrich, St Louis, MO) and α-smooth muscle actin (1:250, ab7817, Abcam, Cambridge, MA) through the use of a Ventana BenchMark GX automated slide staining system (Ventana Medical Systems, Tucson, AZ) in accordance with the instructions of the manufacturer.

Techniques: Histopathology, Staining